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KMID : 1041219840260010035
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Journal of Sericultural and Entomological Science
1984 Volume.26 No. 1 p.35 ~ p.51
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Studies on the Varietal Differences of the Electrophoretic Protein Patterns in the Pupal Haemolymph of the Silkworm, Bombyx mori
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Abstract
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In order to know the similarity of the electrophoretic protein patterns, distributional aspects of the frequency in each protein band, differences of protein patterns between female and male, and the number of the total protein bands of each variety in the haemolymph of the silkworm pupae according to their geographical origin, the silkworm varieties, and both the sexes, variable electrophoretic protein patterns were observed by polyacrylamide gel electrophoresis, using the silkworm strains which have been reared in the sericultural experiment station.
1. The total number of electrophoretic protein bands classified were twenty·eight, and nearly fourteen bands were shown by each variety on an average.
2. According to their geographical origin, the mean values of the similarity were 20.0%, 28.3% and 14.3% in Japanese, Chinese and European respectively, and the highest similarity was observed between Chinese varieties.
3. The similarity of 129 varieties were tested and it was 22.5%, 23.5% in female and male respectively.
4. The electrophoretic protein patterns in 129 varieties were tested and variation was found in 109 patterns in female and 112 in male.
5. The frequency of the each specified protein band in female and male having variation in protein bands in each variety was found averagely 6.6%.
6. The distribution frequency of the classified 28 protein bands in both the sexes within each geographical origin was variable from 0% to 100%.
7. Among the classified 28 protein bands, HP 11 protein band was found common in all the varieties tested.
8. HP 3 and HP 4 protein bands in female showed high density in electropherograms than in male.
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